ISOLATION OF MESENCHYMAL STEM CELLS FROM PERIPHERAL BLOOD
Privatdozent Dr. med. dent. Aydin Gülses, Staatliche Krankenhausvereinigung - Gesundheitsministerium der Republik Türkei
Associate Professor DDS PhD Aydin Gülses, Public Hospital Association - Ministry of Health of the Republic of Turkey
Assoc Prof. Dr. Aydin Gülsesa, Prof rer. nat. Yahya Açil, Prof. Dr. Dr. Jörg Wiltfang
Reconstructive management of craniomaxillofacial bone deficiencies remains still challenging. In the past, several techniques have been described for the isolation of mesenchymal stem cells (MCs) from various tissues/organs. Despite the presence of drawbacks and potential risks regarding the general anethesia and the invasive surgical intervention protocol, the use of bone aspirates collected from the iliac crest is still the most accepted method for optimizing bone volume in craniomaxillofacial reconstruction. 1In order to overcome the above mentioned disadvantages, current study aimed to the demonstrate a novel technique by using peripheral blood samples for isolation of MSCs and define its potential superiority over the previously described methods.
Material and methods
Peripheral blood samples taken from healthy individuals twice in a row will be included in the study. They will be subdivided into two groups regarding the protocol used. In the study group, the samples will be used for direct isolation of the MSCs, whereas the other samples will be managed by the FICOLL protocol. After cell transplantation the viability of the cells and their proliferation capacities will be sustained. Following this, FACS analysis method will be used to isolate the MSCs. For this purpose, required surface antigens will be supplied by the Department of Immunology of Christian Albrechts University.
Potential influential observations