We apply high-throughput methods to define molecular subtypes and characterize disease states in acute leukemias:
Transcriptome Sequencing (mRNA-Seq, total RNA-Seq)
Whole Exome- / Targeted Gene Panel Sequencing
Analysis of chromatin states using ChIP-Seq and PLAC-Seq
DNA-Methylation Chip Arrays
Integrative data analyses pipelines
Single-cell resolution methods are applied to study intra-tumoral heterogeneity and evolutionary trajectories:
Single-Cell RNA-Seq
Cytof
Conventional molecular genetic methods are used for more targeted approaches:
PCR / qRT-PCR / Sanger Sequencing
Multiplex Ligation-dependent Probe Amplification
We create functional models of candidate alterations using:
CRIPR/Cas9 genome editing
Molecular Cloning and overexpression systems
RNA-interference using siRNA and shRNA
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Disease phenotypes and functional regulations are explored in cell line models and primary cultures using:
High-throughput Drug Sensitivity Profiling
Flow Cytometry and Immunophenotyping
Western blotting
Metabolic Profiling
- PCR / qRT-PCR / Sanger Sequencing
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