Research Activities and Methods

We apply high-throughput methods to define molecular subtypes and characterize disease states in acute leukemias:

  • Transcriptome Sequencing (mRNA-Seq, total RNA-Seq)

  • Whole Exome- / Targeted Gene Panel Sequencing

  • Analysis of chromatin states using ChIP-Seq and PLAC-Seq

  • DNA-Methylation Chip Arrays

  • Integrative data analyses pipelines

Single-cell resolution methods are applied to study intra-tumoral heterogeneity and evolutionary trajectories:

  • Single-Cell RNA-Seq

  • Cytof

Conventional molecular genetic methods are used for more targeted approaches:

  • PCR / qRT-PCR / Sanger Sequencing

  • Multiplex Ligation-dependent Probe Amplification

We create functional models of candidate alterations using:

  • CRIPR/Cas9 genome editing

  • Molecular Cloning and overexpression systems

  • RNA-interference using siRNA and shRNA

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Disease phenotypes and functional regulations are explored in cell line models and primary cultures using:

  • High-throughput Drug Sensitivity Profiling

  • Flow Cytometry and Immunophenotyping

  • Western blotting

  • Metabolic Profiling

    • PCR / qRT-PCR / Sanger Sequencing
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